In the fifth plague, God goes further. It involves their most valued possession.

..................It didn't end when Pharaoh said "NO!" to God's request to let his children go; nor when he said "Yes" but didn't keep his promise.

...........................Pharaoh was warned,
"If thou refuse to let them go and wilt hold them still, behold the hand of the Lord is laid upon thy cattle which is in the field, upon the horses, upon the asses, upon the camel, and upon the sheep: There shall be a grievous murrain [animal disease]."
...........................Exodus 9:1-3